Effect of Stem Cell-Derived Extracellular Vesicles on Damaged Human Corneal Endothelial Cells

Figure 1

Isolation and characterization of corneal endothelial cells. (a) Representative micrograph of HCEC at passage 3. (b) Representative micrographs of western blot on four HCEC independent cell lines deriving from different patients (C1, C7, C18, and C23). The control (Ctl) is represented by the renal HK2 cell line, negative for Na + K + ATPase and positive for ZO-1. Actin was used as an endogenous loading reference. (c) Representative flow cytometry analysis of HCECs showing the expression of CD166, CD105, CD29, CD90, CD73, CD34, EPCAM, and Vimentin on HCECs.

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