A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension

Figure 4

Cyclic tension influences BMSC arrangement and morphology. (a) Morphology and arrangement of BMSCs observed under the 4x and 10x microscopes after 6 h, 12 h, 24 h, 36 h, and 48 h of cyclic tension compared with those of the control group (without loading). Scale bars: 500 μm/200 μm. (b) Cell length of BMSCs measured under the microscope after 6 h, 12 h, 24 h, 36 h, and 48 h of cyclic tension compared with that of the control group. and . (c) The green fluorescence indicates the F-actin. The blue fluorescence indicates the cell nucleus. Representative photographs of the control and cyclic tension groups at 6 h, 12 h, 24 h, 36 h, and 48 h under 10x and 20x microscopy are shown. Scale bars: 100 μm/50 μm.

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