A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension

Figure 5

Cyclic tension promotes BMSC osteoblastic and fibroblastic differentiation. The mRNA expression levels of RUNX2 (a) and OCN (b) in response to cyclic tension force under different tension force values (including the control or with extension rates of 9%, 12%, and 15%) for 12 h. The mRNA expression levels of RUNX2 (c), COL-1 (d), OPN (e), SCX (f), COL-3 (g), TN-C (h), IL-6 (i), and IL-1β (j) in response to cyclic tension force with a 12% extension rate for the control or 6 h, 12 h, 24 h, 36 h, or 48 h of loading. Gene expression was calibrated using the GAPDH housekeeping gene. Data represent the ; , , , and vs. the control. (h) Representative chemiluminescent images of western blot analysis of RUNX2, COL-1, OPN, SCX, COL-3, TN-C, and GAPDH in BMSCs. (l-q) Western blot analysis of RUNX2 (l), COL-1 (m), OPN (n), SCX (o), COL-3 (p), and TN-C (q). Gray value was calibrated using GAPDH. Data represent the ; , , , and vs. the control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)

(p)

(q)