Research Article
A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension
Table 1
mRNA primer sequences used in this study.
| | Gene | Forward sequence (5⟶3) | Reverse sequence (5⟶3) |
| | GAPDH | GCATTGCCCTCAACGACCACT | CCATGAGGTCCACCACCCTGT | | RUNX2 | ACTACCAGCCACCGAGACCA | ACTGCTTGCAGCCTTAAATGACTCT | | OCN | AGCAAAGGTGCAGCCTTTGT | GCGCCTGGGTCTCTTCACT | | COL-1 | TGGACGCCATCAAGGTCTACTGC | GGAGGTCTTGGTGGTTTTGTATTCG | | OPN | CGAGGTGATAGTGTGGTTTATGG | GCACCATTCAACTCCTCGCT | | SCX | CAGCCCAAACAGATCTGCACCTT | CTGTCTTTCTGTCGCGGTCCTT | | COL-3 | TGGTCTGCAAGGAATGCCTGGA | TCTTTCCCTGGGACACCATCAG | | TN-C | GTCACCGTGTCAACCTGATG | GTTAACGCCCTGACTGTGGT | | IL-6 | AGACAGCCACTCACCTCTTCAG | TTCTGCCAGTGCCTCTTTGCTG | | IL-1β | TGGCAACTGTTCCTG | GGAAGCAGCCCTTCATCTTT |
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GAPDH: glyceraldehyde 3-phosphate dehydrogenase; RUNX2: Runt-related transcription factor 2; OCN: osteocalcin; COL-1: collagen, type I, alpha 1; OPN: osteopontin; SCX: Scleraxis; COL-3: collagen, type I, alpha 3; TN-C: Tenascin C.
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