Research Article

A Novel Tension Machine Promotes Bone Marrow Mesenchymal Stem Cell Osteoblastic and Fibroblastic Differentiation by Applying Cyclic Tension

Table 1

mRNA primer sequences used in this study.

GeneForward sequence (5⟶3)Reverse sequence (5⟶3)

GAPDHGCATTGCCCTCAACGACCACTCCATGAGGTCCACCACCCTGT
RUNX2ACTACCAGCCACCGAGACCAACTGCTTGCAGCCTTAAATGACTCT
OCNAGCAAAGGTGCAGCCTTTGTGCGCCTGGGTCTCTTCACT
COL-1TGGACGCCATCAAGGTCTACTGCGGAGGTCTTGGTGGTTTTGTATTCG
OPNCGAGGTGATAGTGTGGTTTATGGGCACCATTCAACTCCTCGCT
SCXCAGCCCAAACAGATCTGCACCTTCTGTCTTTCTGTCGCGGTCCTT
COL-3TGGTCTGCAAGGAATGCCTGGATCTTTCCCTGGGACACCATCAG
TN-CGTCACCGTGTCAACCTGATGGTTAACGCCCTGACTGTGGT
IL-6AGACAGCCACTCACCTCTTCAGTTCTGCCAGTGCCTCTTTGCTG
IL-1βTGGCAACTGTTCCTGGGAAGCAGCCCTTCATCTTT

GAPDH: glyceraldehyde 3-phosphate dehydrogenase; RUNX2: Runt-related transcription factor 2; OCN: osteocalcin; COL-1: collagen, type I, alpha 1; OPN: osteopontin; SCX: Scleraxis; COL-3: collagen, type I, alpha 3; TN-C: Tenascin C.