Age-Related Macular Degeneration (AMD) Transmitochondrial Cybrids Protected from Cellular Damage and Death by Human Retinal Progenitor Cells (hRPCs)
Table 3
qRT-PCR analysis for differential gene expression, mitochondrial copy number, and cellular viability for AMD/hRPC vs. AMD and hRPC/AMD vs. hRPC/normal cybrids.
AMD cybrids+hRPCs (Tx) vs. AMD cybrids (CTRL)
hRPCs+AMD cybrids (Tx) vs. hRPC+normal cybrids (CTRL)
Gene
Function
Tx vs. CTRL fold
Tx vs. CTRL value
Gene
Function
hRPC only
hRPC+normal cybrids
hRPC+AMD cybrids
Tx vs. CTRL fold
Tx vs. CTRL value
BAX
Apoptosis
0.78
0.023
MAP2
Neuro. marker
2.08
0.0015
CASP3
Apoptosis
0.95
0.400
GFAP
Glial marker
7.93
<0.0001
CASP7
Apoptosis
0.73
0.0001
MDK
Neuroprotect.
3.53
<0.0001
CASP9
Apoptosis
0.97
0.686
PTN
Neuroprotect.
2.71
<0.0001
ATG5
Autophagy
0.88
0.042
FGF2
Neuroprotect.
2.16
<0.0001
ATG12
Autophagy
0.78
0.068
LAMP2
Autophagy
0.75
0.029
LC3B
Autophagy
0.78
0.014
PARK2
Autophagy
0.74
0.001
DDIT3
ER stress
0.54
0.003
XBP1
ER stress
0.72
0.0001
GPX
Antioxidant
0.74
0.001
SOD2
Antioxidant
0.86
0.047
NQO1
Antioxidant
1.29
0.389
POLG
Mito. repl.
0.73
0.015
POLRMT
Mito. repl.
0.79
0.107
TFAM
Mito. repl.
0.73
0.025
Mito. copy #
1.34
0.361
Cell viability
1.27
0.0019
with 10 values for each sample. . Fold values above 1 indicate upregulation of the gene compared to control. Fold values below 1 indicate downregulation of the gene compared to control. Controls are assigned a value of 1. Comparisons were done using one-sample -tests. AMD: age-related macular degeneration; hRPC: human retinal progenitor cells; Tx: treatment; CTRL: control.
