Therapeutic Effects of Conditioned Medium of Neural Differentiated Human Bone Marrow-Derived Stem Cells on Rotenone-Induced Alpha-Synuclein Aggregation and Apoptosis

Figure 1

(a) SH-SY5Y cells incubated with the absence or presence of ROT (0.5 μM) for 48 h were treated with hBMSC-CM or NI-hBMSC-CM at 100 or 50 or 25% during the last 24 h, and cell survival was assessed by the trypan blue assay. Data are the of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. Statistical significance: ^acompared with control; ^bcompared with ROT; and . (b) Cells incubated with the absence or presence of ROT (0.5 μM) for 48 h were treated with hBMSC-CM (50%) or NI-hBMSC-CM (50%) during the last 24 h and assessed for TH and GAPDH by Western blotting. Each picture is representative of three independent experiments. Data are the of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. Statistical significance: ^acompared with control; ^bcompared with ROT; .

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