Therapeutic Effects of Conditioned Medium of Neural Differentiated Human Bone Marrow-Derived Stem Cells on Rotenone-Induced Alpha-Synuclein Aggregation and Apoptosis

Figure 3

SH-SY5Y cells were seeded as cells/mL of DMEM containing 1% FBS and used for experiments after overnight incubation. Cells incubated with the absence or presence of ROT (0.5 μM) for 48 h were treated with NI-hBMSC-CM (50%) during the last 24 h. Cell lysates were prepared as 1% Triton X-100-soluble and Triton X-100-insoluble (2x SDS soluble) fractions. p-S129 and total α-syn were analyzed from 1% Triton X-100-insoluble (2x SDS soluble) fractions by Western blotting using 12% and 8% SDS-PAGE gels (a). Each picture is representative of three independent experiments. Bar graphs represent fold changes in p-S129 α-syn/GAPDH (b, d) and total α-syn/GAPDH (c, e) in SDS-PAGE gels of 12% (b, c) or 8% (d, e). Data are the of three independent experiments and analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test. Statistical significance: ^acompared with control; ^bcompared with ROT; , , and .

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