Research Article

Dual Effects of Hypoxia-Inducible Factors-1 Alpha in Bleomycin-Induced Pulmonary Fibrosis Treated by Human Umbilical Cord Mesenchymal Stem Cells

Figure 1

Detection of surface markers, osteogenesis, adipogenesis, and cartilage-induced differentiation of HUCMSCs. FACS was used to detect the surface markers, and ICC was used to detect the differentiation. (a–c) The expression of positive surface marker CD73, CD90, and CD105. (d–h) The expression of surface negative markers CD14, CD19, CD34, CD45, and HLA-DR. (i) The osteogenic differentiation was analyzed by Alizarin Red staining. (j) The adipogenic differentiation was analyzed by Oil Red O staining. (j) The chondrogenic differentiation was analyzed by Alcian Blue staining. The μm in (i, j) and 100 μm in (k).
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