HGF-Modified Dental Pulp Stem Cells Mitigate the Inflammatory and Fibrotic Responses in Paraquat-Induced Acute Respiratory Distress Syndrome

Figure 5

DPSCs-HGFs inhibit the fibrotic response in BEAS-2B and MRC-5 cells. (a) The expression of fibrotic factors, including α-SMA, vimentin, Col I, TIMP, and MMP-9, in MRC-5 cells stimulated with TGF-β was measured by qPCR. (b) The expression of fibrotic factors, including α-SMA, VIM, E-cadherin, and MMP-9, in BEAS-2B cells stimulated with TGF-β was measured by qPCR. Each experiment was performed in triplicate; the data are shown as the . ANOVA followed by Tukey’s multiple comparisons test was used to evaluate these data. ^#, ^###, and ^#### vs. Con; , , , and vs. TGF-β; ^&, ^&&&, and ^&&&& vs. DPSCs-null. Con: control; DPSCs: dental pulp mesenchymal stem cells; α-SMA: α-smooth muscle actin; Col I: collagen type I; TIMP: tissue inhibitor of metalloproteinase; MMP-9: matrix metalloproteinase 9.

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