Nsd2 Represses Endogenous Retrovirus MERVL in Embryonic Stem Cells

Figure 2

Depletion of Nsd2 in ESCs. (a) qPCR analysis of the relative expression levels of Nsd2 after Nsd2 depletion in ESCs with normalization to Gapdh. Data were plotted as , biological repeats. (b) Cell morphology of Nsd2 depletion in ESCs. Scale bar, 100 μm. (c) qPCR analysis of pluripotent genes (Oct4, Sox2, and Nanog) in ESCs with Nsd2 depleted. (d–g) qPCR analysis of endoderm markers Foxa2 and Sox17 (d), mesoderm markers Gata4 and Nkx2.5 (e), ectoderm markers Msx1 and Pax6 (f), and trophectoderm markers Foxd3 and Gata3 (g). (h) qPCR analysis of the expression levels of MERVL and other retrotransposons (LINE1, SINE B1) after Nsd2 depletion in ESCs. The results were normalized to Gapdh. Data were shown as ; biologically independent replicates; ns: nonsignificant; ; ; .

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