Lin28 Inhibits the Differentiation from Mouse Embryonic Stem Cells to Glial Lineage Cells through Upregulation of Yap1

Figure 1

Constitutive expression of Lin28a/b in mouse ESC induced Yap1 expression and reduced lineage-specific gene expression. (a) Phase-contrast microscopy and AP staining of Ctrl and Lin28a/b constitutively expressed (Lin28a/b OE) mouse ESCs grown under 2i + LIF medium. Scale bar, 200 μm. (b) Western blot analyses of total proteins from Ctrl and Lin28a/b OE mouse ESCs using the indicated antibodies. Oct4, Sox2, and Nanog are pluripotent stem cell markers. (c) Quantitative real-time PCR to examine the mRNA level of lineage-specific gene expression in Ctrl and Lin28a/b OE mouse ESCs, trophectoderm gene Cdx2, ectoderm gene Nestin, mesoderm gene T, and endoderm gene Gata6. Actin was analyzed as an internal control. The data are shown as the (). Statistically significant differences were indicated (, and , ). (d) Western blot analyses of total proteins from Ctrl and Lin28a/b OE mouse ESCs using the indicated antibodies. (e) Quantitative real-time PCR to examine the mRNA level of Yap1 and its downstream target gene Ctgf expression in Ctrl and Lin28a/b OE mouse ESCs. The data are shown as the ().

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