Lin28 Inhibits the Differentiation from Mouse Embryonic Stem Cells to Glial Lineage Cells through Upregulation of Yap1

Figure 3

Constitutive overexpression of Lin28a/b inhibited the differentiation of mouse ESCs to glial lineage cells. (a) Western blot analyses of total proteins from Ctrl and Lin28a/b OE mouse ESC differentiated cells on day 5 using the indicated neural stem cell markers: Sox1, Nestin, and Sox2. (b) Western blot analyses of total proteins from Ctrl and Lin28a/b OE mouse ESC differentiated cells on day 10 using the indicated neuronal marker: β-tubulin III, glial markers: Gfap and Mbp. (c) Western blot analyses of total proteins from Ctrl and Lin28a/b OE mouse ESC differentiated cells on day 15 using the indicated neuronal subtype markers: Th, vGlut2, Gad1, and Chat. (d) Immunofluorescence staining of the Ctrl and Lin28a/b OE mouse ESC differentiated cells on day 15 using the neuronal marker β-tubulin III and glial markers Gfap and Mbp, and neuronal subtype markers Th. Cell nuclear was stained with DAPI. Scale bar, 200 μm.

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