Research Article

Lin28 Inhibits the Differentiation from Mouse Embryonic Stem Cells to Glial Lineage Cells through Upregulation of Yap1

Figure 5

Inhibition of Yap1-Tead interaction using verteporfin partially rescued the glial cell lineage differentiation defect of Lin28a/b OE mouse ESCs. (a) Western blot analyses of total proteins from Ctrl mouse ESCs treated with different concentrations of Yap1-Tead interaction inhibitor (verteporfin) using the indicated antibodies. (b) A schematic drawing of the differentiation assay from neural stem cells (day 5) to neuronal and glial lineage cells (day 15) combining with verteporfin. (c) Western blot analyses of total proteins from Ctrl and Lin28a OE mouse ESC differentiated cells (treated with DMSO and Verteporfin, respectively) on day 10 using the indicated antibodies. (d) Western blot analyses of total proteins from Ctrl and Lin28a OE mouse ESC differentiated cells (treated with DMSO and verteporfin, respectively) on day 15 using the indicated antibodies. (e) Immunofluorescence staining of the Ctrl and Lin28a OE mouse ESC differentiated cells (treated with DMSO and verteporfin, respectively) on day 15 using the neuronal marker β-tubulin III, glial markers Gfap and Mbp, and neuronal subtype markers chat. Cell nuclear was stained with DAPI. Scale bar, 200 μm.
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