Continuous Inhibition of Sonic Hedgehog Signaling Leads to Differentiation of Human-Induced Pluripotent Stem Cells into Functional Insulin-Producing <i>β</i> Cells

<div>Spheroid formation and sustained maintenance of IPCs. (a) Many small clusters of IPCs form after day 1 of IPC dissociation, and numerous spheroid IPCs are generated after 5 d. (b) Many cells express insulin or glucagon in spheroid IPCs. (c) Levels of insulin are decreased in differentiated IPCs cultured with improved MEM (1× B27) that was used for differentiation after continuous culture for 10 d. Cells stably secrete insulin in culture medium containing 10% FBS.</div>

Stem Cells International

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Figure 6

Figure 6: Continuous Inhibition of Sonic Hedgehog Signaling Leads to Differentiation of Human-Induced Pluripotent Stem Cells into Functional Insulin-Producing <i>β</i> Cells 

Figure 6 | Continuous Inhibition of Sonic Hedgehog Signaling Leads to Differentiation of Human-Induced Pluripotent Stem Cells into Functional Insulin-Producing β Cells 