Research Article

Proteomic Analysis Reveals Commonly Secreted Proteins of Mesenchymal Stem Cells Derived from Bone Marrow, Adipose Tissue, and Synovial Membrane to Show Potential for Cartilage Regeneration in Knee Osteoarthritis

Table 1

The list of commonly secreted proteins identified by all mesenchymal stem cells (MSCs) under normal and inflammatory conditions.
(a)

Identified proteins of normal condition (105 proteins)
AccessionDescriptionGene symbol

A0A024QYT5Serpin peptidase inhibitor, clade ESERPINE1
A0A024R1U8Insulin-like growth factor-binding protein 4IGFBP4
A0A024R2W4Dystroglycan 1DAG1
A0A024R462Fibronectin 1FN1
A0A024R6R4Matrix metallopeptidase 2MMP2
A0A024R8V7TIMP metallopeptidase inhibitor 2TIMP2
A0A024RDW8Collagen, type IV, alpha 2COL4A2
A0A087WTA8Collagen alpha-2(I) chainCOL1A2
A0A087X0S5Collagen alpha-1(VI) chainCOL6A1
A0A0A0MT01GelsolinGSN
A0A0F7G8J1PlasminogenPLG
A0A140VJI7Testicular tissue protein Li 61ECM1
A0A161I202LactoferrinLTF
A0A172Q381EndosialinCD248
A1L4H1Soluble scavenger receptor cysteine-rich domain-containing protein SSC5DSSC5D
A4D2D2Procollagen C-endopeptidase enhancerPCOLCE
A6XND1Insulin-like growth factor-binding protein 3IGFBP3
A8K2H4Cathepsin BCTSB
A8K7Q1Nucleobindin 1NUCB1
A8KAJ3EGF-containing fibulin-like extracellular matrix protein 1EFEMP1
B2R582C-type lectin domain family 3, member BCLEC3B
B2R5M9Procollagen-lysine, 2-oxoglutarate 5-dioxygenasePLOD1
B2RBS8AlbuminALB
B2RCM5EG-containing fibulin-like extracellular matrix protein 2EFEMP2
B2RDW0Calmodulin 2 (phosphorylase kinase, delta)CALM2
B4DDQ2BiglycanBGN
B4DNG0Olfactomedin-like protein 3OLFML3
B4DPH4PlasminogenPLG
B4DPQ0Complement C1r subcomponentC1R
B4DPZ5Polymerase I and transcript release factorPTRF
B4DU16Fibronectin 1FN1
B4E3Q1Calsyntenin-1CLSTN1
D0PNI2Lysyl oxidaseLOX
D1MGQ2Alpha-2 globin chainHBA2
D3DTX7Collagen, type I, alpha 1COL1A1
D3YTG3Target of Nesh-SH3ABI3BP
D6RF35Vitamin D-binding proteinGC
D9ZGG2VitronectinVTN
F8VR42Dynein regulatory complex subunit 2CCDC65
F8W6I7Heterogeneous nuclear ribonucleoprotein A1HNRNPA1
H0YGS3Microfibrillar-associated protein 5MFAP5
H7BZJ3Protein disulfide-isomerase A3PDIA3
H7C0V9Amyloid-beta A4 proteinAPP
I4AY87Macrophage migration inhibitory factorMIF
O00391Sulfhydryl oxidase 1QSOX1
P00338L-Lactate dehydrogenase A chainLDHA
P00441Superoxide dismutase [Cu-Zn]SOD1
P01023Alpha-2-macroglobulinA2M
P01024Complement C3C3
P01033Metalloproteinase inhibitor 1TIMP1
P01034Cystatin-CCST3
P02452Collagen alpha-1(I) chainCOL1A1
P02461Collagen alpha-1(III) chainCOL3A1
P02647Apolipoprotein A-IAPOA1
P0538760S acidic ribosomal protein P2RPLP2
P05997Collagen alpha-2(V) chainCOL5A2
P07585DecorinDCN
P07737Profilin-1PFN1
P07942Laminin subunit beta-1LAMB1
P08670VimentinVIM
P09382Galectin-1LGALS1
P09486SPARCSPARC
P09871Complement C1s subcomponentC1S
P10599ThioredoxinTXN
P11047Laminin subunit gamma-1LAMC1
P12110Collagen alpha-2(VI) chainCOL6A2
P14543Nidogen-1NID1
P18206VinculinVCL
P21333Filamin-AFLNA
P24592Insulin-like growth factor-binding protein 6IGFBP6
P26022Pentraxin-related protein PTX3PTX3
P30041Peroxiredoxin-6PRDX6
P35442Thrombospondin-2THBS2
P35555Fibrillin-1FBN1
P36955Pigment epithelium-derived factorSERPINF1
P48061Stromal cell-derived factor 1CXCL12
P50454Serpin H1SERPINH1
P51884LumicanLUM
P55290Cadherin-13CDH13
P63261Actin, cytoplasmic 2ACTG1
P63313Thymosin beta-10TMSB10
Q01995TransgelinTAGLN
Q08629Testican-1SPOCK1
Q0Z944Beta globinHBB
Q12841Follistatin-related protein 1FSTL1
Q14767Latent-transforming growth factor beta-binding protein 2LTBP2
Q15582Transforming growth factor-beta-induced protein ig-h3 1TGFBI
Q16270Insulin-like growth factor-binding protein 7IGFBP7
Q16778Histone H2B type 2-EHIST2H2BE
Q53FA4Cysteine-rich, angiogenic inducer, 61 variantCYR61
Q53G99Beta actin variantACTB
Q59GA0Thy-1 cell surface antigen variantTHY1
Q5M8T4Connective tissue growth factorCTGF
Q6EMK4VasorinVASN
Q6FHC9STC2 proteinSTC2
Q6FHW3DF proteinCFD
Q6IAW5CALU proteinCALU
Q6YHK3CD109 antigenCD109
Q8IUX7Adipocyte enhancer-binding protein 1AEBP1
Q99715Collagen alpha-1(XII) chainCOL12A1
V9HWC6Peptidyl-prolyl cis-trans isomerasePPIB
V9HWE8Epididymis secretory sperm binding protein Li 47eARHGDIA
V9HWI5Cofilin 1 (nonmuscle)CFL1
V9HWK1Triosephosphate isomeraseTPI1
V9HWN7Fructose-bisphosphate aldolaseALDOA

(b)

Identified proteins of inflammatory condition (93 proteins)
AccessionDescriptionGene symbol

A0A024R1U8Insulin-like growth factor binding protein 4IGFBP4
A0A024R462Fibronectin 1FN1
A0A024R5Z7AnnexinANXA2
A0A024R6R4Matrix metallopeptidase 2MMP2
A0A024R8V7TIMP metallopeptidase inhibitor 2TIMP2
A0A024R969Chitinase 3-like 1CHI3L1
A0A024RDA5Multifunctional fusion proteinIL-8; CXCL8
A0A087WTA8Collagen alpha-2(I) chainCOL1A2
A0A087X0S5Collagen alpha-1(VI) chainCOL6A1
A0A0A0MT01GelsolinGSN
A0A0S2Z3G9Actinin alpha 4 isoform 1ACTN4
A0A161I202LactoferrinLTF
A0A1B0GU92Uncharacterized proteinN/A
A0A1U9X7H4CFBCFB
A4D1W7Inhibin, beta A (activin A, activin AB alpha polypeptide)INHBA
A4D2D2Procollagen C-endopeptidase enhancerPCOLCE
A6XND1Insulin-like growth factor binding protein 3 isoform bIGFBP3
A8K7Q1Nucleobindin 1NUCB1
B2R4R0Histone H4HIST1H4A
B2R5J8C-C motif chemokineCCL5
B2RBS8AlbuminALB
B2RCM5EGF-containing fibulin-like extracellular matrix protein 2EFEMP2
B2RDW0Calmodulin 2CALM2
B3KQT9Protein disulfide-isomerasePDIA3
B4DDQ2BiglycanBGN
B4DLV7Rab GDP dissociation inhibitorGDI2
B4DMR3Glia-derived nexinSERPINE2
B4DPQ0Complement C1r subcomponentC1R
B4E324Lysosomal protective proteinCTSA
B4E3Q1Calsyntenin-1CLSTN1
B5MCZ3Interleukin-6IL-6
D0PNI1Epididymis luminal protein 4YWHAZ
D3DTX7Collagen, type I, alpha 1COL1A1
D3YTG3Target of Nesh-SH3ABI3BP
D6RF92C-X-C motif chemokineCXCL6
D9ZGF2Collagen, type VI, alpha 3COL6A3
F8W6I7Heterogeneous nuclear ribonucleoprotein A1HNRNPA1
I4AY87Macrophage migration inhibitory factorMIF
O00300Tumor necrosis factor receptor superfamily member 11BTNFRSF11B
O00391Sulfhydryl oxidase 1QSOX1
P01024Complement C3C3
P01033Metalloproteinase inhibitor 1TIMP1
P01034Cystatin-CCST3
P02461Collagen alpha-1(III) chainCOL3A1
P04083Annexin A1ANXA1
P07585DecorinDCN
P07737Profilin-1PFN1
P07996Thrombospondin-1THBS1
P08254Stromelysin-1MMP3
P08670VimentinVIM
P09341Growth-regulated alpha proteinCXCL1
P09382Galectin-1LGALS1
P09486SPARCSPARC
P09871Complement C1s subcomponentC1S
P10599ThioredoxinTXN
P11047Laminin subunit gamma-1LAMC1
P12110Collagen alpha-2(VI) chainCOL6A2
P13500C-C motif chemokine 2CCL2
P14543Nidogen-1NID1
P15018Leukemia inhibitory factorLIF
P18206VinculinVCL
P19875C-X-C motif chemokine 2CXCL2
P19876C-X-C motif chemokine 3CXCL3
P20809Interleukin-11IL-11
P21333Filamin-AFLNA
P24592Insulin-like growth factor-binding protein 6IGFBP6
P26022Pentraxin-related protein PTX3PTX3
P35442Thrombospondin-2THBS2
P35555Fibrillin-1FBN1
P48307Tissue factor pathway inhibitor 2TFPI2
P51884LumicanLUM
P62328Thymosin beta-4TMSB4X
P63313Thymosin beta-10TMSB10
P98066Tumor necrosis factor-inducible gene 6 proteinTNFAIP6; TSG-6
Q12841Follistatin-related protein 1FSTL1
Q16270Insulin-like growth factor-binding protein 7IGFBP7
Q16778Histone H2B type 2-EHIST2H2BE
Q53G71Calreticulin variantCALR
Q53G75Matrix metalloproteinase 1 preproprotein variantMMP1
Q53G99Beta actin variantACTB
Q53GY0Plastin 3 variantPLS3
Q6EMK4VasorinVASN
Q6FHZ0Malate dehydrogenaseMDH2
Q6IAW5CALU proteinCALU
Q75MU2Uncharacterized protein WBSCR1EIF4H
Q86Z22Epididymis secretory protein Li 297SH3BGRL3
Q8IUX7Adipocyte enhancer-binding protein 1AEBP1
Q99715Collagen alpha-1(XII) chainCOL12A1
V9HWB4Epididymis secretory sperm binding protein Li 89nHSPA5
V9HWC6Peptidyl-prolyl cis-trans isomerasePPIB
V9HWI5Cofilin 1CFL1
V9HWN7Fructose-bisphosphate aldolaseALDOA
V9HWP2Epididymis luminal protein 35HSP90B1