Adult Human Multipotent Neural Cells Could Be Distinguished from Other Cell Types by Proangiogenic Paracrine Effects via MCP-1 and GRO

Figure 1

Proangiogenic activities of ahMNCs. (a) In vitro tube formation assay. Different ratios of CM of ahMNCs were applied to HUVECs in Matrigel for 18 h. Tube length and mesh area were analyzed and compared. Scale bar, 200 μm. . . Representative data (001TL) of triplicated experiments are shown. (b) In vivo Matrigel plug assay. cells at ratios of 100 : 0, 50 : 50, or 0 : 100 (HUVECs : fNSCs or HUVEC : ahMNCs) in Matrigel were transplanted subcutaneously into dorsal surfaces of Balb-c/nu mice. Four days after transplantation, in vivo angiogenesis was analyzed histologically. Scale bar, 100 μm. Representative data (015TL) of triplicated experiments are illustrated. (c, d) After transplantation of ahMNCs tagged with GFP and HUVEC (c), or ahMNCs and HUVEC tagged with GFP (d), their localization was analyzed by immunofluorescence. Scale bars, 20 μm. Representative data (001TL) of triplicated experiments are illustrated.

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