Spidroin-Based Biomaterials in Tissue Engineering: General Approaches and Potential Stem Cell Therapies

Figure 4

Adhesion of different cells on spider silk fibers. (a) Representative phase contrast versus confocal and immunostaining images of (A) rSCs, (B) rFBs, and (C) rDRG neurons cultured on spider silk. The merged channels of rSCs were S100 (green), VIME (grey), and DAPI (blue); the merged channels of rFBs were NGFR (magenta), THY1 (cyan), and DAPI (grey); and the merged channels of rDRG neurons were TUJ1 (beta-3-tubulin) (green), NGFR (magenta), and DAPI (blue). Arrows (left) indicate elongated rDRGs along the silk fibers, and arrows (right) indicate rFBs and rDRG neuron bodies. Reproduced from F. Millesi et al., 2021, under the Creative Commons Attribution License. (b) Images showed the proliferation and alignment of DPSCs on the spider silk over time (10x magnification). Reproduced from K. Hafner et al., 2017, Copyright © 2021, Elsevier B.V. All rights reserved. (c) Immunostaining images of rSCs cultured on (A and B) PLL/laminin-coated dishes and (C) dragline, (D) cocoon, (E) connecting, and (F) attaching silk showing S100 (green) and DAPI (blue) staining. The white arrow points towards the single silk strands that were barely perceivable. Reproduced from A. Naghilou et al., 2020, under the Creative Commons Attribution License.

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