miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3

<div>Dkk3 is a direct target of miR-129-5p. (a) A diagram showed potential targets of miR-129-5p analyzed by TargetScan, miRDB, and miRWalk. (b) Bioinformatics prediction of miR-129-5p binding sites in the 3<svg height="11.4859pt" id="M65" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>UTR of Dkk3 mRNA. (c) Luciferase activities of reporters containing the 3<svg height="11.4859pt" id="M66" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -11.4361 3.89517 11.4859" width="3.89517pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M310 541L304 571C290 586 211 619 185 610L80 76L131 52L310 541Z"></path></g></svg>UTR of Dkk3 in miR-129-5p-transduced, miR-129-5p-mutant-transduced, or negative control 293T cells. The relative luciferase activity in 293T cells cotransfected with negative control was designated as 1. (d) Protein levels of Dkk3 were examined by western blot. (e) Representative image of immunofluorescence staining with Dkk3 (red) antibody and DAPI (blue) in BMSCs transduced with the indicated lentivirus. Scale bars, 25 <i>μ</i>m. 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Stem Cells International

fig6

Figure 6

Figure 6: miR-129-5p Promotes Osteogenic Differentiation of BMSCs and Bone Regeneration via Repressing Dkk3 