Research Article
lncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived from Adipose-Derived Stem Cells for the Treatment of Osteoporosis
Figure 2
Knockdown of miR-141-5p reverses the effect of TNF-α on primary osteoblasts. (a) anti-miR-141-5p reduced the expression of miR-141-5p more significantly in primary osteoblasts compared to anti-miR-NC. (b) CCK-8 assays revealed that the knockdown of miR-141-5p partly reversed the inhibition of cell viability induced by TNF-α when cells were treated with PBS, TNF-α, TNF-α+anti-miR-NC, TNF-α+anti-miR-141-5p (1μg), or TNF-α+anti-miR-141-5p (2 μg), respectively. (c) The increase of Bax and cleaved-Caspase-3 expression induced by TNF-α was inhibited by downregulating miR-141-5p. (d) Annexin V-FITC/PI revealed that the knockdown of miR-141-5p partly reversed cell apoptosis induced by TNF-α. , , and .
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