Research Article

lncRNA-KCNQ1OT1: A Potential Target in Exosomes Derived from Adipose-Derived Stem Cells for the Treatment of Osteoporosis

Figure 5

KCNQ1OT1-Exos inhibit TNF-α-induced cytotoxicity and apoptosis of primary osteoblasts. (a, b) The expression of KCNQ1OT1 in ADSCs or exosomes derived from the ADSCs after different transfections was elevated, but the expression of miR-141-5p was downregulated compared with that in LV-NC-treated group after the transfection of LV-KCNQ1OT1. (c) KCNQ1OT1 expression was upregulated in primary osteoblasts treated with LV-KCNQ1OT1-Exos compared to NC-Exos. (d) In primary osteoblasts, LV-KCNQ1OT1-Exos mitigated the negative effect of TNF-α on cell viability, while ADSCs-Exos exerted a weaker stimulative effect on cell viability compared to LV-KCNQ1OT1-Exos. (e) When primary osteoblasts were cocultured with LV-KCNQ1OT1-Exos, the TNF-α-induced cell apoptosis was reversed and ADSCs-Exos exerted a weaker inhibitory effect on cell apoptosis compared to LV-KCNQ1OT1-Exos. (f) The expression of Bax and cleaved caspase-3 in primary osteoblasts was blocked after coculture of LV-KCNQ1OT1-Exos. , , and .
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