Chondrogenic Potential of Human Dental Pulp Stem Cells Cultured as Microtissues

Figure 5

Immunofluorescence microphotographs of hDPSC microtissue cultures with chondrogenic differentiation medium for (a–d) 2 weeks, (e–h) 4 weeks, and (i–l) 6 weeks. Control samples were incubated only with the secondary antibody. Cellular nuclei are observed in blue, and the presence of the different specific macromolecules was analysed in green. To facilitate the visualization of the fluorescence signal, green channel parameters of some images were modified by PhotoShop: (a, c) and and (e–g) and . .