Research Article
Melatonin Alleviates LPS-Induced Pyroptotic Cell Death in Human Stem Cell-Derived Cardiomyocytes by Activating Autophagy
Figure 4
Melatonin reduced cell pyroptosis in LPS-exposed hiPSC-CMs. (a) Representative images of flow cytometry results. (b) Quantitative analysis of pyroptosis rate. (c) Representative immunoblots of NLRP3, cleaved casp-1, and GSDMD-NT. (d) Quantitative analysis of NLRP3. (e) Quantitative analysis of cleaved casp-1. (f) Quantitative analysis of GSDMD-NT. (g) Representative immunoblots of cleaved IL-1β and cleaved IL-18. (h) Quantitative analysis of cleaved IL-1β. (i) Quantitative analysis of cleaved IL-18. (j) IL-1β level examined by ELISA assay. (k) IL-18 level examined by ELISA assay. ; vs. control; vs. control; # vs. LPS; ## vs. LPS.
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