Gli1⁺ Cells Residing in Bone Sutures Respond to Mechanical Force via IP₃R to Mediate Osteogenesis

Figure 4

Mechanical stretching regulates JBMMSC osteogenesis via IP₃R-mediated intracellular calcium increases. (a) Intracellular calcium concentrations visualized at mean fluorescence intensity decreased after 2-APB treatment, compared with the stretch group. ; . (b) JBMMSCs treated with 2-APB showed fewer mineralized nodules, here stained using alizarin red. (c) The abundance of mineralized nodules in the stretch group was higher than that in the stretch+2-APB group. ; . (d) Western blot analysis indicated downregulation of ALP, RUNX2, and Col1 protein expression in JBMSCs after 2-APB and mechanical stretching treatment, compared with cells subjected only to mechanical stretching. β-Tubulin was used as an internal control; . (e) RT-qPCR showed downregulation of ALP, RUNX2, and Col1 RNA expression in JBMSCs after 2-APB and mechanical stretching treatment, compared with cells subjected only to mechanical stretching. GAPDH was used as an internal control. ; ^ns; .

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