Gli1⁺ Cells Residing in Bone Sutures Respond to Mechanical Force via IP₃R to Mediate Osteogenesis

Figure 6

Gli1⁺ cells participate in RME-induced bone formation via IP₃R upregulation. (a) Distribution of IP₃R⁺ cells (red), Gli1⁺ cells (green), and IP₃R⁺ and Gli1⁺ cells in the control, RME, and RME+GANT61 groups detected with immunofluorescence staining. Scale bar: 100 μm. Regions in boxes are magnified in the right panel: “N” indicates the nasal side of the midpalatal suture and “O” indicates the oral side. Scale bar: 50 μm; . (b) IP₃R⁺ cells were increased in the RME group and decreased after GANT61 treatment. ; ; . (c) IP₃R⁺ and Gli1⁺ cells increased in the RME group and decreased after GANT61 treatment. ; ; .

(a)

(b)

(c)