Research Article
Gli1+ Cells Residing in Bone Sutures Respond to Mechanical Force via IP3R to Mediate Osteogenesis
Table 1
Primers used for gene typing.
| | Gene type | Forward primer sequence (5–3) | Reverse primer sequence (5–3) |
| | Gli1-LacZ (wild type) | GGGATCTGTGCCTGAAACTG | AGGTGAGACGACTGCCAAGT | | Gli1-LacZ (mutant type) | GGGATCTGTGCCTGAAACTG | TCTGCCAGTTTGAGGGGACGAC | | Gli1-CreERT2 (wild type) | GCGGTCTGGCAGTAAAAACTATC | GTGAAACAGCATTGCTGTCACTT | | Gli1-CreERT2 (mutant type) | GGGATCTGTGCCTGAAACTG | CTTGTGGTGGAGTCATTGGA | | mT/mG (wild type) | CTCTGCTGCCTCCTGGCTTCT | CGAGGCGGATCACAAGCAATA | | mT/mG (mutant type) | CTCTGCTGCCTCCTGGCTTCT | TCAATGGGCGGGGGTCGTT |
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