Gli1⁺ Cells Residing in Bone Sutures Respond to Mechanical Force via IP₃R to Mediate Osteogenesis

Table 2

Antibodies used in this research.

Antibody	Manufacture	Catalogue number	Concentration	Application
Anti-GAPDH	Yesen Biotechnology	30201ES20	1 : 2000	WB
Anti-beta tubulin	Proteintech	10068-1-AP	1 : 2000	WB
Anti-RUNX2	Cell Signaling Technology	12556	1 : 200 for IF; 1 : 1000 for WB	IF; WB
Anti-β-gal	Abcam	ab9361	1 : 200	IF
Anti-IP3R	Abcam	ab108517	1 : 200 for IF; 1 : 1000 for WB	IF; WB
Anti-ALP	Santa Cruz Biotechnology	sc-79840	1 : 1000	WB
Anti-Col1	Abcam	ab34710	1 : 1000	WB
Anti-CD73	eBioscience	12-0731	1 : 100	FC
Anti-CD105	BioLegend	120413	1 : 100	FC
Anti-CD29	eBioscience	17-0291-82	1 : 100	FC
Anti-CD11b	BioLegend	101227	1 : 100	FC
Anti-CD45	BD Pharmingen	553134	1 : 100	FC
Anti-sca-1	eBioscience	17-5981	1 : 100	FC