Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in Chemotherapy-Induced POF Rats

Figure 1

Identification and differentiation potential of stem cells. The surface markers and trilineage differentiation potential of the stem cells were tested. (a) The HucMSCs were positive for CD44, CD73, CD90, and CD105 and negative for CD34, CD45, CD11b, CD14, CD19, CD79α, and HLA-DR. (b) Morphology of the stem cells under a light microscope. Scale bar: 200 μm, 50 μm. (c) Alizarin Red staining of undifferentiated stem cells. Scale bar: 500 μm, 100 μm. (d) Oil Red O staining of undifferentiated stem cells. Scale bar: 500 μm, 100 μm. (e) Alcian blue staining of undifferentiated stem cells. Scale bar: 500 μm, 100 μm. (f) After 21 days of incubation in osteogenic differentiation medium, red calcium nodules appeared on Alizarin Red staining. Scale bar: 500 μm, 100 μm. (g) After 21 days of incubation in adipogenic differentiation medium, red fat droplets appeared in stem cells stained with Oil Red O. Scale bar: 500 μm, 100 μm. (h) After 21 days of incubation in the chondrogenic differentiation medium, the acid mucopolysaccharide was stained blue with Alcian blue staining. Scale bar: 500 μm, 100 μm.

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