Research Article

Activated Human Umbilical Cord Blood Platelet-Rich Plasma Enhances the Beneficial Effects of Human Umbilical Cord Mesenchymal Stem Cells in Chemotherapy-Induced POF Rats

Figure 2

The effect of ucPRP on the proliferation and apoptosis of the HucMSCs. (a) EdU fluorescence after incubation of ucPRP and FBS for 24 hours. Red represents DNA replication, and blue represents the cell nucleus. Scale bar: 500 μm, 100 μm. (b) Numbers of replicated cells were quantified for the indicated groups. (c) Flow cytometry to detect the apoptosis of HucMSCs after incubation with ucPRP and FBS. (d) After 24 hours of stimulation, flow cytometry showed that ucPRP reduced the early apoptosis of HucMSCs. (e) After 24 hours of stimulation, flow cytometry showed that ucPRP reduced the late apoptosis of HucMSCs. (f) After 24 hours of stimulation, flow cytometry showed that ucPRP reduced the total apoptosis of HucMSCs. Data represent the . ++, +++.
(a)
(b)
(c)
(d)
(e)
(f)