RAB37 Promotes Adipogenic Differentiation of hADSCs via the TIMP1/CD63/Integrin Signaling Pathway

Figure 6

Knockdown of CD63 compromised RAB37-mediated proliferation and adipogenic differentiation. The lentivirus expressing shRNA targeting CD63 (shCD63) was used to inhibit endogenous expression of CD63 in RAB37-ADSCs. Lentivirus expressing a scramble RNA was used as control. Adipogenic differentiation was evaluated by Oil Red O staining (a); . Oil Red O-stained hADSCs in six random views were counted (b), . The expression of TIMP1 (c), PPARγ (d), C/EBPα (e), and LPL (f) was examined by qRT-PCR, . Data are presented as . ; lv-scramble vs. lv-shCD63.

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