ALK5 i II Accelerates Induction of Adipose-Derived Stem Cells toward Schwann Cells through a Non-Smad Signaling Pathway

Figure 2

ALK5 i II prompted hADSCs to express SC markers. Human ADSCs were seeded in 24-well plates and cultured in a complete medium or a SC medium supplemented with the compounds indicated. Various signal pathway inhibitors (a, b) and TGFβR inhibitors (c) were compared, while various concentrations of ALK5 i II were also tested (d). After 14 days of culture, RNA was extracted from the cells and subjected to real-time RT-PCR analysis. Each dot represents triplicate value of relative mRNA level for the indicated genes. and vs. ADSCs cultured in the SC medium. ^## vs. all the other groups. ⁺⁺ vs. ADSCs cultured in the SC medium supplemented with ALK5 i II at 0, 0.25, or 1 μM. n.s.: no significant difference between the indicated groups. Experiments were repeated three times.

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