ALK5 i II Accelerates Induction of Adipose-Derived Stem Cells toward Schwann Cells through a Non-Smad Signaling Pathway

Figure 3

Comparison of relative mRNA levels among ADSCs, cSCLCs, mSCLCs, and gSCs. Cells were seeded into 24-well plates and cultured in the indicated medium for 14 days. (a) RNA was extracted from the cells, and real-time RT-PCR was performed to evaluate mRNAs for the indicated genes. Each dot represents triplicate value. vs. ADSCs. ^## vs. mSCLCs. ⁺⁺ vs. gSCs. (b) ADSCs as negative control and cSCLCs, mSCLCs, and gSCs as positive control were stained with the indicated antibodies, while cell nuclei were stained with Hoechst. Fluorescence microscopic images (magnification: ×200) are shown. . Experiments were repeated three times.

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