ALK5 i II Accelerates Induction of Adipose-Derived Stem Cells toward Schwann Cells through a Non-Smad Signaling Pathway

Figure 5

Neither Smad2 nor Smad3 were indispensable for induction of ADSCs into mSCLCs. ADSCs were transduced with lentiviral vectors encoding sh-NC, sh-Smad2, and/or sh-Smad3 as indicated. Forty-eight hours later, selection of transfected cells was started by adding puromycin and/or blasticidin. Stable transfectants were treated with BME and ATRA as indicated and cultured in the indicated culture medium as in Figure S1. After 14 days of culture, RNA was extracted from the cells and subjected to real-time RT-PCR to determine mRNA levels of the indicated genes. Each dot represents a triplicate value. vs. sh-NC. n.s.: no significant difference between the indicated groups.