Research Article
Therapeutic Effect of Stem Cells on Male Infertility in a Rat Model: Histological, Molecular, Biochemical, and Functional Study
Figure 3
Photomicrographs of H&E-stained sections from the rat testis. (a) Control testis showing normal seminiferous tubules lined by spermatogonia (SG), lying on regular basement membranes (BM), primary spermatocytes (SP), rounded spermatids (SD), mature sperms (S) inside the lumen, Sertoli cells (SC), interstitial tissues containing Leydig cells (L), and flat nuclei of myoid cells (M). (b) MTX group showing disruption of seminiferous tubules (ST) with complete loss of spermatogenic cells and Sertoli cells and some tubules with sloughing of germ cells into the tubular lumens with loss of sperms (arrowheads). Congested blood vessels (C), tunica albuginea (TA), and oedema in interstitial tissue (IF). (c) MTX group showing complete loss of spermatogenic cell lines and Sertoli cells (arrows), cells having nuclei with chromatin margination (arrowheads), vacuolations (V) with loss of mature sperms, and oedema in interstitial tissue (IF). (d) ADMSC-treated testis showing normal seminiferous tubules and myoid cells (M), lined by normal spermatogonia (SG), spermatocytes (P), spermatids (SP), sperms (S), and triangular Sertoli cell (SC) resting on the intact basement membrane. Interstitial tissues contain Leydig cells (L) (H&E ×400, photo inside frame, ×100).