Human ES Cell Culture Conditions Fail to Preserve the Mouse Epiblast State

Figure 1

Human ESC/EpiSC culture condition leads naïve ESCs to distinct EpiSC-like states. (a) Brightfield images of the indicated samples (scale bars 100 μm). mESCs were brought to naïve state with LIF/2i (LIF+2i (PD0325901+CHIR99021)), followed by AA/F2 treatment. This treatment results in morphological changes from round dome-shaped to flat colonies. (b) Principal component analysis (PCA) of the indicated samples. (c) Heat map, based on Pearson’s correlation coefficient. AA/F2 treatment for different durations led naïve ESCs to primed EpiSC states that differed from each other and the PSCs that were compared here.

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