|
| Cell types | Main induction methods | Achievements | Journal, year (reference) |
| Generated cells | Characterization of generated cells |
|
| ESCs | Spontaneous differentiation
Suspension culture | Oocyte-like cells | Morphology
Marker expressions (SCP1, SCP3, and GDF9) | Hum Mol Genet, 2004 [61] |
| ESCs | BMP4
Suspension culture | PGCs | Morphology
Marker expressions (VASA and SCP3) | Stem cells dev, 2006 [62] |
| VSELs | Spontaneous differentiation
Suspension culture | Oocyte-like cells | Morphology
Marker expressions (C-KIT, VASA, and ZP2) | Differentiation, 2008 [78] |
ESCs
iPSCs | Coculture with fetal gonadal cells
Adherent culture | PGCs | Morphology
Marker expressions (DAZL, VASA, and SSEA1) | Stem Cells, 2009 [12] |
| ESCs | VASA overexpression
BMP4, BMP7, and BMP8b
Adherent culture | PGCs | Morphology
Marker expressions (DAZL, VASA, and SCP3)
Epigenetic analysis | Nature, 2009 [66] |
| ESCs | RA
Suspension culture | Oocyte-like cells | Morphology
Marker expressions (SSEA1, DAZL, and VASA) | Hum Repro, 2009 [63] |
| iPSCs | Overexpression of DAZL and BOULE
BMP4, BMP7, and BMP8b
Adherent culture | PGCs | Morphology
Marker expressions (STELLA and DMC1)
Elongated SC formation | Human Mol Genet, 2011 [68] |
| VSELs | Spontaneous differentiation
Adherent culture | Oocyte-like cells | Morphology
Marker expressions (DAZL, ZP4, and GDF9) | Stem Cells Dev, 2011 [79] |
ESCs
iPSCs | VASA overexpression
Adherent culture | PGCs
Postmeiotic GCs | Morphology
Marker expressions (GCNF, LHR, and ZP2)
SCP formation analysis
Epigenetic analysis | Stem Cells, 2012 [67] |
| ESCs | STELLA overexpression
RA
Adherent culture | PGCs | Marker expressions (VASA, SCP3, and SOX17) | PloS one, 2013 [69] |
ESCs
iPSCs | (i) 4i (MAPK, GSK3, P38, and JNK inhibitors), LIF, TGFβ, and bFGF
Adherent culture
(ii) BMP2/4, LIF, SCF, and EGF
Suspension culture | PGCLCs | Morphology
Marker expressions (BLIMP1 and STELLA)
Global transcription profiles
Epigenetic analysis | Cell, 2015 [7] |
| iPSCs | (i) ActA and GSK3b inhibitor
Adherent culture
(ii) GMEM/KSR, BMP4, LIF, SCF, and EGF
Suspension culture | iMeLCs
PGCLCs | Morphology
Marker expressions (PRDM14 andSOX17)
Global transcription profiles
Epigenetic analysis | Cell stem cell, 2015 [6] |
ESCs
iPSCs | (i) ActA, bFGF, and BMP4 (5 ng/ml)
Adherent culture (ii) Lif and BMP4 (100 ng/ml)
Suspension culture | Mesodermal-like cells
PGCLCs | Morphology
Marker expressions (BLIMP1 and STELLA) global transcription profiles
Epigenetic analysis | EMBO J, 2015 [36] |
| ESCs | Overexpression of DAZL and BOULE
GDF9 and BMP15
Adherent culture | FLCs | Morphology
Marker expressions (ZP2, NOBOX, and AMH)
Global transcription profiles
Estradiol secretion | Nat commun, 2017 [74] |
| VSELs | Follicular fluid “serum” medium
Adherent culture | Oocyte-like cells | Morphology
Marker expression (ZP1-3) | Stem Cell Rev Rep, 2018 [80] |
| iPSCs | Coculture with mouse gonadal cells
Suspension culture | PGCLCs
Oogonia-like cells | Morphology
Marker expressions (SCP3, REC8 and STRA8)
Transcriptome dynamics
Epigenetic analysis
X chromosome activity | Science, 2018 [8] |
|
