Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation

Figure 2

Effect of L. japonicum Thunb. fruit crude extract (LJE) on the viability of hBM-MSCs (a). Cells were seeded ( cells/well) in 96-well plates and incubated for 72 h untreated or treated with different concentrations (10 and 100 μg/ml) of LJE. Viability of the cells was measured by quantification of MTT dye removed from cells. Cell viability is given as relative percentage of untreated control. Effect of LJE on the activity of cellular ALP (b). Cellular ALP activity of osteo-induced hBM-MSCs was measured with a spectrophotometric enzymatic activity assay at day 7 of differentiation. Effect of LJE on the intracellular lipid accumulation of adipo-induced hBM-MSCs (c). Following 14 days of differentiation, lipid droplets in adipo-induced hBM-MSCs were visualized with Oil Red O staining. Images of cells show stained lipid droplets (upper panel). Lipid accumulation levels were calculated by the colorimetric quantification of the dye removed from the wells (lower panel) and given as percentage of adipo-induced untreated control group. and vs. the untreated differentiated control group. Scale bar: 50 μm.

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