Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation

Figure 6

Effect of salidroside on the osteogenic differentiation of hBM-MSCs. Viability of the (a) noninduced and (b) osteo-induced cells was measured after 72 h and 5 days, respectively. Cells were seeded () in 96-well plates with different concentrations (0, 1, 5, and 10 μM) of salidroside and 10% DMSO as vehicle (0, 0.01, 0.05, and 0.25% final concentrations). Cell viability was compared to untreated control kept in culture medium only and given as relative percentage (a). hBM-MSCs were osteo-induced at 100% confluence without or with salidroside treatment (1, 5, and 10 μM) (b). Effect of salidroside (1, 5, and 10 μM) on the cellular ALP activity of osteo-induced hBM-MSCs was measured with a spectrophotometric enzymatic activity assay at day 7 of differentiation (c). Effect of salidroside on extracellular calcification was examined in osteo-induced hBM-MSCs without or with salidroside (1, 5, and 10 μM) treatment (d). At day 14 of differentiation, hBM-MSCs were stained with Alizarin Red. Images of cells show stained calcium deposits (upper panel). Mineralization levels were calculated by the colorimetric quantification of the dye removed from the wells (lower panel) and given as percentage of the untreated differentiated control group. and vs. the untreated differentiated control group. Scale bar: 25 μm.

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