Ligustrum japonicum Thunb. Fruits Exert Antiosteoporotic Properties in Bone Marrow-Derived Mesenchymal Stromal Cells via Regulation of Adipocyte and Osteoblast Differentiation

Figure 8

Effect of salidroside on the adipogenic differentiation of hBM-MSCs. (a) Adipo-induced hBM-MSCs in 6-well plates without or with salidroside treatment (1, 5, and 10 μM) were stained with Oil Red O at day 14 of differentiation. Images of cells show stained lipid droplets (upper panel). Lipid accumulation levels were calculated by the colorimetric quantification of the dye removed from the wells (lower panel) and given as percentage of the untreated differentiated control group. Analysis of (b) mRNA and (c) protein levels was carried out by RT-PCR and Western blotting, respectively, using the adipo-induced hBM-MSC lysates at day 14 of differentiation. PCR and protein bands were densitometrically quantified and plotted as relative percentage of the untreated differentiated control group. β-Actin was used as internal loading control. and vs. the untreated differentiated control group. Scale bar: 50 μm.

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