Different Neurogenic Potential in the Subnuclei of the Postnatal Rat Cochlear Nucleus

Figure 3

Neural stem cells of all CN subnuclei can differentiate into the cell forms of the neuroectodermal line. (a–c) Undifferentiated cells showed a positive staining for the progenitor cell marker nestin. (d–f) Neuronally differentiated cells were identified by β-III-tubulin staining (red). These cells had slim, spindle-shaped, neuron-typical somata with bi- and multipolar formations. (g–i) Astrocytes were identified by GFAP staining (glial fibrillary acidic protein). These cells showed an astrocyte-typical stellate-shaped formation with multipolar branches. (j–l) Oligodendrocytes were stained with MBP (myelin basic protein). These showed a beginning myelinization of the peripheral processes, while the still unmyelinated cell bodies were stained β-tubulin (β-T) positive. Cell nuclei were stained with DAPI.