HLA DR Genome Editing with TALENs in Human iPSCs Produced Immune-Tolerant Dendritic Cells

Figure 3

Characterization of wild-type and HLA DR knockout iPSCs. Both wild-type and HLA DR knockout iPSCs show similar stemness potential. (a) Alkaline phosphatase staining of WT and HLA DR knockout iPS colonies. (b) Immunostaining of iPSCs for pluripotency-related genes Nanog, Oct4, and SSEA4. (c) Teratoma formation showed that wild-type and HLA DR knockout iPSCs differentiated into three germ layers of tissues in vivo (scale bars: 200 μm) and (d) compared the expression of HLA DR expression in CD45-positive tissues (scale bars: 200 μm). Only wild type teratomas showed HLA DR expression in CD45-positive tissues.

(a)

(b)

(c)

(d)