Expression Profile of Long Noncoding RNAs and Circular RNAs in Mouse C3H10T1/2 Mesenchymal Stem Cells Undergoing Myogenic and Cardiomyogenic Differentiation

Figure 2

The morphology and characterization of mouse MSCs and the relative expression profile of cardiomyogenic and myogenic marker genes. Up: 5-azacytidine induces C3H10T1/2 cells into differentiated myocytes. (a) C3H10T1/2 cells. (b) C3H10T1/2 cells were treated with 20 μM 5-azacytidine for 7 days, and cells were displayed in the same direction. (c and d) Myotube-like structure appeared after 5-azacytidine treating, then cells were changed into growth media and cultured for the next 21 days. (e and f) Differentiated myocytes were picked with cell ring and cultured for additional two weeks. Myocyte-like cells could be subcloned and had abilities to form myotube-like structure. Down: the relative expression profile of cardiomyogenic and myogenic marker genes: GATA-4, cTnt, MEF2C, NKX-2.5, MyoD, Myogenin, Desmin, and α-MHC were determined using real-time PCR at 0, 7, and 21 days after 5-azacytidine treatment (, ).