Expression Profile of Long Noncoding RNAs and Circular RNAs in Mouse C3H10T1/2 Mesenchymal Stem Cells Undergoing Myogenic and Cardiomyogenic Differentiation

Figure 5

Differential expression profiles of mRNA-lncRNAs between undifferentiated and differentiated MSCs. (a) The volcano figure of the differentially expressed mRNAs between 5-AZA-treated and untreated cells, respectively (C3h10_1 and C3h10_2: control; C3h10_101 and C3h10_102: case). (b) Differential gene expression profiling was identified by hierarchical clustering analysis, indicating that undifferentiated and differentiated stem cells exhibited dramatically differentially expressed gene profile (C3h10_1 and C3h10_2: control; C3h10_101 and C3h10_102: case). (c) The volcano figure of the differentially expressed lncRNAs between 5-AZA-treated and untreated cells, respectively (C3h10_1 and C3h10_2: control; C3h10_101 and C3h10_102: case). (d) Differential lncRNA profiling was identified by hierarchical clustering analysis, indicating that undifferentiated and differentiated stem cells exhibited a dramatically lncRNA expression profile (C3h10_1 and C3h10_2: control; C3h10_101 and C3h10_102: case). (e) Statistics of lncRNA types showed that there are (i) 240 intergenic lncRNAs (u), (ii) 197 intronic lncRNAs (i), (iii) 105 anti-sense lncRNAs (x), (iv) 365 sense-overlapping lncRNA intergenic (o).

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