Intestinal Organoids in Colitis Research: Focusing on Variability and Cryopreservation

Figure 6

Cryopreservation affects gene expression levels in colonoids after EcN infection. The gene expression levels in freshly isolated colonoids () and cryopreserved and thawed organoids () of the same passage after infection with EcN were compared. The graphs plot the pooled technical replicate means of biological replicates plus the overall mean and 95% CI. , , , and . (a) Relative Ocln expression (ANOVA: ; ). (b) Relative Zo-1 expression (ANOVA: ; ). (c) Relative Cldn4 expression (ANOVA: ; ). (d) Relative Tnfα expression (ANOVA: ; ).

(a)

(b)

(c)

(d)