Optimal Pore Size of Honeycomb Polylactic Acid Films for In Vitro Cartilage Formation by Synovial Mesenchymal Stem Cells

Figure 5

Histological images and thickness of cartilage derived from synovial MSCs plated on PLA films. (a) Histological images. MSCs ( in 500 μL medium) were plated, cultured in chondrogenic induction medium for 14 days, and stained with safranin O. The results of three different samples are shown. (b) Method for measuring the cartilage thickness. A single line was drawn along the long axis of the cartilage, the midpoint of both ends of the cartilage was determined on this line (point A), and seven perpendicular lines were drawn every 500 μm on both sides from “point A.” The midpoints of both ends of the cartilage were determined on each vertical line (M1-M7), and the minimum width through these points was determined. (c) Average thickness of the cartilage and the coefficient of variance for cartilage thickness at 7 points. Means and standard deviations are shown (). . values were determined by a paired Student’s test.

(a)

(b)

(c)