3D Spheroid Formation Using BMP-Loaded Microparticles Enhances Odontoblastic Differentiation of Human Dental Pulp Stem Cells

Figure 3

Odonto/osteoblastic differentiation of 3D spheroid-cultured hDPSCs improves by the sustainably released BMP-2 and BMP-4. (a) Odonto/osteoblastic marker gene expression of hDPSC spheroids. (A) Osterix. (B) Dentin matrix protein-1. (C) Osteocalcin. (D) Bone sialoprotein. (E) Collagen type-1. ; ; ; NS: not significant. (b) Calcium deposition in spheroids. 30 spheroids were collected to analyze the amounts of calcium as followed in Experimental procedures. White bars, cell numbers in spheroids containing particles without BMPs; black bars, cell numbers in spheroids containing particles loaded BMPs. ; ; . (c) Immunohistochemical staining of hDPSCs spheroids for two representative odontoblastic markers such as bone sialoprotein (A) and dentin sialophosphoprotein (B). The paraffin section of hDPSC-spheroids collected at an indicated time was treated with the primary antibody, followed by FTIC-conjugated secondary antibody. LSS: spheroids containing particles without BMPs; LSS/BMPs: spheroids containing particles loaded BMPs.

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