3D Spheroid Formation Using BMP-Loaded Microparticles Enhances Odontoblastic Differentiation of Human Dental Pulp Stem Cells

Figure 4

Comparison of odonto/osteoblastic differentiation of 2D monolayer-cultured hDPSCs and 3D spheroid-cultured hDPSCs. (a) Daily treat amounts of BMP-2 (A) and BMP-4 (B) in 2D monolayered culture. hDPSCs on culture dish were treated with BMPs once every 3 days. Based on the release behavior from LSS particles (Figure 1(b)), the daily average value was calculated from the sum of three days’ values and displayed on the graph as a point. (b) Odonto/osteoblastic marker gene expression in hDPSCs cultured in 2D monolayer and in 3D spheroids. (A) Osteopontin. (B) Osterix. (C) Dentin matrix protein-1. (D) Osteocalcin. (E) Bone sialoprotein. (F) Collagen type-1. ; ; ; NS: not significant. (c) Calcium deposition in hDPSCs cultured in 2D monolayer and in 3D spheroids. 30 spheroids were collected to analyze the amounts of calcium as followed in Experimental procedures. White bars, 2D monolayer culture treated with BMPs; black bars, 3D spheroids containing particles loaded BMPs. ; ; NS: not significant.

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