Research Article

Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis

Figure 2

Mesenchymal evolution along HIO passaging. HIOs stained for the epithelial marker, E-cadherin, and the mesenchymal marker, vimentin, in three different passages (a). Vimentin-positive staining area is shown to be reduced through subsequent passages, suggesting that the mesenchymal component is gradually decreased (b). Representative 40x immunofluorescence snapshots are shown.
(a)
(b)