Development of a Human Intestinal Organoid Model for <i>In Vitro</i> Studies on Gut Inflammation and Fibrosis

<div>Expression of mesenchymal markers and ECM components during passaging. The mRNA levels of CD90, collagen types I and III, and fibronectin were gradually reduced after passage 2 (a–d), with the exception of <i>α</i>-SMA, which showed a tendency to increase during passages 4-6, but later decreased to basal levels (e). ND: nondetectable. All experiments were performed in triplicate. The gene expression of each studied gene was normalized against GAPDH gene expression in the same sample using the 2<sup>-<i>ΔΔ</i>Ct</sup> method. Passage 1 expression levels were set as a reference point, and expression levels in later passages were compared to that. 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Stem Cells International

fig3

Figure 3

Figure 3: Development of a Human Intestinal Organoid Model for <i>In Vitro</i> Studies on Gut Inflammation and Fibrosis 

Stem Cells International

Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis

Figure 3