Research Article
Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis
Figure 3
Expression of mesenchymal markers and ECM components during passaging. The mRNA levels of CD90, collagen types I and III, and fibronectin were gradually reduced after passage 2 (a–d), with the exception of α-SMA, which showed a tendency to increase during passages 4-6, but later decreased to basal levels (e). ND: nondetectable. All experiments were performed in triplicate. The gene expression of each studied gene was normalized against GAPDH gene expression in the same sample using the 2-ΔΔCt method. Passage 1 expression levels were set as a reference point, and expression levels in later passages were compared to that. Data are presented as the .
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