Research Article

Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis

Figure 4

HIOs exhibit fibrotic responses to inflammatory cytokines. IL-1α and TNF-α (2C) induced the mRNA expression of α-SMA in passage 4 (a), fibronectin in passages 4 and 8 (b), TF in passage 4 (c), collagen type I in passages 2 and 4 (d), and collagen type III in passages 2, 4, and 6 (e). Concentrations of cytokines used: IL-1α 5 ng/ml, TNF-α 50 ng/ml. All experiments were performed in triplicate. The gene expression of each studied gene was normalized against GAPDH gene expression in the same sample using the 2-ΔΔCt method. In every passage, expression levels of treated organoids were normalized against those of the control ones. Data are presented as the .
(a)
(b)
(c)
(d)
(e)