Development of a Human Intestinal Organoid Model for <i>In Vitro</i> Studies on Gut Inflammation and Fibrosis

<div>HIOs exhibit mesenchymal and epithelial chemokine protein responses to inflammatory cytokines. IL-1<i>α</i> and TNF-<i>α</i> (2C) 24 h and 48 h stimulation induced the protein expression of CXCL1 (b), CXCL8 (c), CCL2 (d), and CCL20 (e) in passages 2 and 4, while CXCL10 was induced only in passage 2 (a). CCL20 was also induced in passage 6, after 48 h stimulation. Concentrations of cytokines used: IL-1<i>α</i> 5 ng/ml, TNF-<i>α</i> 50 ng/ml. All experiments were performed in triplicate. 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Stem Cells International

fig6

Figure 6

Figure 6: Development of a Human Intestinal Organoid Model for <i>In Vitro</i> Studies on Gut Inflammation and Fibrosis 

Stem Cells International

Development of a Human Intestinal Organoid Model for In Vitro Studies on Gut Inflammation and Fibrosis

Figure 6